Composite

Part:BBa_K1253114:Design

Designed by: Edward Hsieh   Group: iGEM14_TAS_Taipei   (2014-06-27)


This part is used to test for TetR half-life.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1484
    Illegal AgeI site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2507


Design Notes

TetR/RFP and GFP work as reporters that allow the user to deduce TetR half-life.


Source

TAS_Taipei iGEM team.

References